Q&A Scientific and Practical Conference “Modern Achievements in Laboratory Diagnostics for Practical Medicine”

Questions for the speaker on the topic “The Evolution of SHOE Methods” Lyudmila Makhmutova

1. What are the preferred methods of the VCA for manual determination of ESR in the absence of controlled material?

The method of random repeated samples, and when examining a stable population – based on the average value of patient results.

2. If blood for ESR determination is collected in EDTA tubes, is it also advisable to add sodium citrate before placing it on the Panchenko stand? 

The Panchenko method involves diluting blood with a sodium citrate solution. The method in a capillary tube with a Panchenko stand for samples without sodium citrate requires separate verification.

3. I am very interested in the ESR on photometric devices (namely Mindray), the norms for devices for different age categories and gender.

According to the available literature, the results closely correlate with the results of the Westergren method. There are few scientific articles so far. Only the manufacturer can provide reference values.

Questions for the speaker on the topic “Interpretation of histograms and scatterograms on a hematology analyzer” by Alexander Melnik

What is the significance of the RDWs indicator if its value is increased (57.8 f/l) with a reference value of 20-42 f/l? Erythrocyte indicators are within the reference values, including the RDWc indicator. 

In this case, in my opinion, we have a “false elevated” RDW. This may be due to high reticulocytosis due to their large volume, which widens the base of the red blood cell distribution curve. The causes of a “false-high” RDW may also include cold agglutinins, diabetic ketoacidosis, plasma hyperosmolarity, hypernatremia, high leukocytosis, prolonged blood storage, and macrothrombocytosis.